Our research Current projects Treating DEB itch with stem cells Professor John McGrath and Professor Jemima Mellerio work at King’s College, London, UK on this project to see if putting bone marrow cells from people who don’t have EB into the blood of people who do have EB can reduce symptoms of itching. The project will compare skin and blood from people with dystrophic EB pruriginosa (a sub type of DEB that causes particularly itchy skin), regular DEB and no EB at all and ask them to complete questionnaires before and after the DEB-p group receive the cell therapy. If the cell therapy works it may be developed into routine clinical care for DEB patients. Contents: Project information Abstract Project: Evaluation of the early efficacy of intravenously administered allogeneic mesenchymal stromal cells on itching in adults with Epidermolysis Bullosa Pruriginosa (PRUMEC - PRUSTEM) Investigator: Professor John McGrath and Professor Jemima Mellerio Institution: Kings College, London - St John’s Institute of Dermatology, Division of Genetics and Molecular Medicine Grant: £497,360 Start Date: 01/05/2018 for 2 and a half years This research grant is divided in to two stages - PRUMEC and PRUSTEM. Stage 1 - PRUMEC Case-control mechanistic study of pruritus (itch) in adults with dystrophic epidermolysis bullosa pruriginosa. Stage 2 - PRUSTEM - Evaluation of the early efficacy of intravenously administered allogeneic mesenchymal stromal cells on itching in adults with Epidermolysis Bullosa Pruriginosa Abstract This research is about itch in Epidermolysis Bullosa (EB). We know that itching is a common and distressing symptom, and that better control of itch is an important research priority for people living with EB. At the moment, it is not understood why the skin is itchy in EB and we know that most current treatments are not particularly effective. Previous clinical trials of a form of cell therapy took bone marrow cells (mesenchymal stromal cells, MSCs) from unrelated healthy donors and infused them via a vein into children and adults with Recessive Dystrophic EB; these trials were called EBSTEM and ADSTEM and showed that the cells were safe and that in most people the cell infusions had positive benefits for wound healing, skin pain and itching. This project will investigate the underlying mechanisms and potential treatment for itch in EB will be divided in to two stages, PRUMEC and PRUSTEM. Stage 1 - PRUMEC This is a case control study, (a type of observational study involving 2 or more groups) which aims to determine whether the groups have different outcomes after the study. As this is a mechanistic study, this means the research aims to understand the biological processes behind pruritus (itch) and identify treatment targets for this. Since this research involves studying the underlying mechanisms of pruritus, there will be 3 distinct groups: 10-30 adults with dystrophic epidermolysis bullosa pruriginosa (DEB-P), which will have skin and blood samples taken and compared to, 10-30 adults with DEB non-pruriginosa subtype (DEB-NP), and 10-30 healthy volunteers as control groups. Recruited patients will be matched if possible, as closely as what is practical clinically and logistically feasible, based on age, gender, ethnicity, EB severity, time of biopsy and site of biopsy. Questionnaires as well as the blood and skin samples will be taken from each group over a 12 month period. This will allow comparison between groups. Stage 2 - PRUSTEM (Phase I/II trial) This stage will follow the completion of PRUMEC, where the sub group of DEB-P patients enrolled will be given a medicinal product depending on the results of the PRUMEC. The blood tests, questionnaires and biopsies will be repeated and carried out as they were in the PRUMEC study and compared with the baseline assessments carried out in the first stage. The medicinal product will be in the form of cell therapy where up to 10 adults with EB pruriginosa will each receive 3 infusions of MSCs. This work will measure the itch using a detailed scoring system called the Leuven Itch Scale, which has been previously tested in EB. Skin and blood will be examined after the cell therapy has been administered to investigate how the cells might be working in reducing itch and compared with results taken before. The results will also be compared with the blood of 10 people with DEB - NP and 10 control subjects who do not have EB from the PRUMEC study. Those individuals will not receive MSC cell therapy, but their samples will be helpful in trying to work out what causes the very itchy EB pruriginosa in the first place. The overall aim of this work is to improve understanding about (a) what causes itch in EB pruriginosa (and potentially other forms of EB), (b) whether intravenous infusions of MSCs offer a helpful treatment for people with EB pruriginosa and, (c) how do the MSCs work in reducing pruritus in EB pruriginosa. It is hoped that the combined results of EBSTEM, ADSTEM and PRUSTEM/PRUMEC will define the potential benefits of this form of cell therapy for people with DEB (and potentially other forms of EB) and provide momentum for the development of MSC cell therapy as part of routine clinical care for DEB. What is important about this research? “One of the most troubling symptoms in people with EB is itch. At the moment, we know little about what causes the itch and even less about how to treat it. We are delighted to receive DEBRA funding to work on itch. We will be studying people with a very itchy form of EB called EB pruriginosa. We also plan to carry out a clinical trial of intravenous bone marrow cells that we hope will lessen the itch and improve EB skin” Investigator Biography John McGrath MD FRCP FMedSci is Professor of Molecular Dermatology at King’s College London and Head of the Genetic Skin Disease Unit, as well as Head Consultant Dermatologist at St John’s Institute of Dermatology, the Guy’s and St Thomas’ NHS Foundation Trust in London. He was previously a DEBRA-funded junior EB research fellow and has worked on EB research for more than 25 years. He now leads and collaborates on several National and International projects to develop gene, cell, protein and drug therapies that can lead to better treatments for people living with EB.